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dc.contributor.authorTaira, Cleison Ledesma
dc.contributor.authorOkay, Thelma Suely
dc.contributor.authorDelgado, Artur Figueiredo
dc.contributor.authorCeccon, Maria Esther Jurfest Rivero
dc.contributor.authorAlmeida, Margarete Teresa Gottardo de
dc.contributor.authorDel Negro, Gilda Maria Barbaro
dc.date.accessioned2024-10-21T18:45:51Z
dc.date.available2024-10-21T18:45:51Z
dc.date.issued2014
dc.identifier.citationBMC Infect. Dis.
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2334-14-406
dc.identifier.urihttps://repositorio.maua.br/handle/MAUA/1480
dc.description.abstractBackground: Nosocomial candidaemia is associated with high mortality rates in critically ill paediatric patients; thus, the early detection and identification of the infectious agent is crucial for successful medical intervention. The PCR-based techniques have significantly increased the detection of Candida species in bloodstream infections. In this study, a multiplex nested PCR approach was developed for candidaemia detection in neonatal and paediatric intensive care patients. Methods: DNA samples from the blood of 54 neonates and children hospitalised in intensive care units with suspected candidaemia were evaluated by multiplex nested PCR with specific primers designed to identify seven Candida species, and the results were compared with those obtained from blood cultures. Results: The multiplex nested PCR had a detection limit of four Candida genomes/mL of blood for all Candida species. Blood cultures were positive in 14.8% of patients, whereas the multiplex nested PCR was positive in 24.0% of patients, including all culture-positive patients. The results obtained with the molecular technique were available within 24 hours, and the assay was able to identify Candida species with 100% of concordance with blood cultures. Additionally, the multiplex nested PCR detected dual candidaemia in three patients. Conclusions: Our proposed PCR method may represent an effective tool for the detection and identification of Candida species in the context of candidaemia diagnosis in children, showing highly sensitive detection and the ability to identify the major species involved in this infection.en
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo(Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP))pt_BR
dc.description.sponsorshipFAPESP
dc.format.extent7.0
dc.languageInglêspt_BR
dc.publisherBMCen
dc.relation.ispartofBMC INFECTIOUS DISEASES
dc.rightsAcesso Aberto
dc.sourceWeb of Scienceen
dc.subjectCandidaemiaen
dc.subjectCandida sppen
dc.subjectMultiplex PCRen
dc.subjectICUen
dc.subjectPaediatricen
dc.subjectDiagnosisen
dc.subjectPOLYMERASE-CHAIN-REACTIONen
dc.subjectINVASIVE CANDIDIASISen
dc.subjectFUNGAL PATHOGENSen
dc.subjectRISK-FACTORSen
dc.subjectWHOLE-BLOODen
dc.subjectDIAGNOSISen
dc.subjectEPIDEMIOLOGYen
dc.subjectSURVEILLANCEen
dc.subjectCULTURESen
dc.subjectSERUMen
dc.subjectInfectious Diseasesen
dc.subject.otherCandidaemiaen
dc.subject.otherCandida sppen
dc.subject.otherMultiplex PCRen
dc.subject.otherICUen
dc.subject.otherPaediatricen
dc.subject.otherDiagnosisen
dc.subject.otherPOLYMERASE-CHAIN-REACTIONen
dc.subject.otherINVASIVE CANDIDIASISen
dc.subject.otherFUNGAL PATHOGENSen
dc.subject.otherRISK-FACTORSen
dc.subject.otherWHOLE-BLOODen
dc.subject.otherDIAGNOSISen
dc.subject.otherEPIDEMIOLOGYen
dc.subject.otherSURVEILLANCEen
dc.subject.otherCULTURESen
dc.subject.otherSERUMen
dc.subject.otherInfectious Diseasesen
dc.titleA multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patientsen
dc.typeArtigo de Periódicopt_BR
dc.identifier.doi10.1186/1471-2334-14-406
dc.description.affiliationUniversidade de Sao Paulo
dc.description.affiliationInstituto Maua de Tecnologia
dc.description.affiliationUniversidade de Sao Paulo
dc.description.affiliationUniversidade de Sao Paulo
dc.description.affiliationUniversidade de Sao Paulo
dc.description.affiliationUniversidade de Sao Paulo
dc.description.affiliationFaculdade de Medicina de Sao Jose do Rio Preto (FAMERP)
dc.identifier.wosWOS:000339525900002
dc.contributor.orcidOkay, Thelma/0000-0002-5985-388X
dc.contributor.orcidAlmeida, Margarete Teresa Gottardo de/0000-0002-8665-9126
dc.contributor.orcidLedesma Taira, Cleison/0000-0001-9803-2366
dc.contributor.orcidDel Negro, Gilda Maria Barbaro/0000-0002-4322-312X
dc.citation.volume14


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